Abstract:

Wu Lining, Li Chen, Wang Dan, Cao Binglan, Zhu Jingxian Department of Dermatology, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China （Wu LN, Zhu JX）; Department of Infectious Diseases, Fifth Hospital of Shijiazhuang, Shijiazhuang 050021, China （Li C）; Department of Dermatology, Affiliated Hospital of Chengde Medical University, Chengde 067000, Hebei, China （Wang D）; Department of Dermatology, Yuanshi County Hospital of Traditional Chinese Medicine, Shijiazhuang 051130, China （Cao BL） Corresponding author: Zhu Jingxian, Email: zhujingxian@medmail.com.cn 【Abstract】 Objective To evaluate in vitro antifungal activities of five clinically common antifungal agents against Geotrichum silvicola, and to observe morphological changes of Geotrichum silvicola after treatment with terbinafine. Methods Five antifungal agents commonly used in clinic, including terbinafine （TER）, amphotericin B （AmB）, flucytosine （FC）, fluconazol （FCZ） and itraconazole （ICZ）, were tested in this study. According to the National Committee for Clinical Laboratory Standards （NCCLS） M27-A2 document and relevant literatures, a susceptibility test was performed to determine the minimal inhibitory concentration （MIC） values of the five antifungal agents against Geotrichum silvicola. Scanning electron microscopy （SEM） was used to observe morphological changes of Geotrichum silvicola treated with different concentrations of TER. Results The MIC values against Geotrichum silvicola were as follows: TER, 0.01 μg/ml; AmB, 0.4 μg/ml; FC, 2 μg/ml; FCZ, 2.69 μg/ml; ICZ, 0.25 μg/ml. Geotrichum silvicola was sensitive to TER, AmB, FC and FCZ, and sensitive to ICZ in a dose-dependent manner. SEM revealed that after TER treatment, the surface of Geotrichum silvicola became rough and shrinking with irregular defects and holes, and even gave a fragment-like appearance. Conclusions Among the five tested antifungal agents, TER shows the strongest activity against Geotrichum silvicola. Within a certain range, the higher the concentration of TER, the more serious the damage to Geotrichum silvicola.