Abstract:

Li Yuping, Zhang Xiaoguang, Guo Bingshen, Bai Yanzhi, Zhang Lihua, Wu Chennan, Li Suyue Department of Dermatology, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, China （the current affiliation of the sixth author was Department of Dermatology, Children′s Hospital Affiliated to Capital Institute of Pediatrics, Beijing 100020, China） Corresponding author: Li Yuping, Email: lyuping@medmail.com.cn 【Abstract】 Objective To evaluate effects of thalidomide on proliferative activity of as well as s of collagenⅠα1 and Ⅲα1 mRNAs and collagenⅠα1 protien in human dermal fibroblasts （HDFs）, and to preliminarily explore the potential role of thalidomide in the fight against dermal fibrosis. Methods HDFs were isolated from the foreskin of a healthy young man, and subjected to primary culture. The fourth?passage HDFs were used in the following experiment. Some HDFs were divided into 3 groups to be treated with 10?3, 10?4 and 10?5 mol/L thalidomide respectively, and other HDFs treated with DMEM alone served as the blank control group. After 24?hour culture, cell counting kit?8 （CCK?8） assay was performed to evaluate the proliferative activity of HDFs, fluorescence?based quantitative PCR to measure the mRNA s of collagen Ⅰα1 and Ⅲα1, and Western?blot analysis to determine the protein of collagen Ⅰα1. Results Compared with the blank control group, the 10?3? and 10?4?mol/L thalidomide groups both showed significantly decreased cell survival rates （77.40% ± 4.25% and 88.56% ± 6.43% vs. 100.00% ± 6.74%, both P < 0.05）, but the 10?5?mol/L thalidomide group showed no significant difference （96.66% ± 1.098%, P > 0.05）. Compared with the blank control group, the 10?3?, 10?4? and 10?5?mol/L thalidomide groups all showed significantly lower mRNA and protein s of collagen Ⅰα1 （mRNA: 0.279 ± 0.025, 0.427 ± 0.040 and 0.658 ± 0.032 vs. 1.016 ± 0.003; protein: 0.279 ± 0.020, 0.926 ± 0.048 and 1.523 ± 0.027 vs. 1.773 ± 0.059; all P < 0.05）. In addition, the mRNA s of collagen Ⅲα1 were also significantly weaker in the 10?3?, 10?4? and 10?5?mol/L thalidomide groups compared with the blank control group （0.551 ± 0.039, 0.756 ± 0.025 and 0.826 ± 0.018 vs. 0.988 ± 0.012, all P < 0.05）. Conclusion Thalidomide within a certain range of concentrations can inhibit the proliferation of HDFs, as well as s of collagenⅠα1 mRNA and protein and collagen Ⅲα1 mRNA.