Abstract: ZHANG Yun-feng*, MIU Fei, WANG Hong-wei, L?譈 Ting, LI Jing-jing, WANG Xiu-li. *Shanghai Skin Disease Hospital, Shanghai 200050, China Corresponding author: WANG Xiu-li, Email: xlwang2001@yahoo.cn 【Abstract】 Objective To investigate the local immune response in condyloma acuminatum treated with aminolevulinic acid-photodynamic therapy （ALA-PDT）. Methods In vitro and in vivo studies were performed. A previously established keratinocyte cell line human papilloma virus （HPV） 16E7/HaCaT which stably expresses HPV16E7 protein was used in this study. Peripheral blood mononuclear cells（PBMCs） were separated from 10 healthy volunteers. After pretreatment with ALA-PDT, HPV16E7/HaCaT cells were cocultured with the PBMCs for 3 hours in a Transwell chamber followed by the observation of chemotactic migration of PBMCs. Tissue samples were obtained from the lesions of 10 patients with condyloma acuminatum before, and at 1, 2, 3 and 48 hours after the first session of ALA-PDT. Immunohistochemistry was conducted to determine the number of CD4＋ T cells, CD8＋ T cells and CD68＋ macrophages as well as CD4/CD8 T-cell ratio in the tissue samples. Results After 3-hour coculture with HPV16E7/HaCaT cells pretreated by ALA-PDT, PBMCs showed apparent chemotactic migration. Immunohistochemistry revealed a statistical increase in the number of CD4＋ T cells , CD8＋ T cells and CD4/CD8 T-cell ratio at 48 hours （all P < 0.05），as well as in the number of CD68＋ macrophages at 3 hours and 48 hours （both P < 0.05） after the first session of ALA-PDT. Conclusion ALA-PDT may induce local antiviral immune response in condyloma acuminatum. 【Key words】 Condylomata acuminata; Aminolevulinic acid; Photochemotherapy; Immunity

Key words: Condyloma Acuminatum, aminolevulinic acid