Abstract: WEN Pei-jun, LIAO Qi-man, LI Wen-jing, CHEN Mu-kai, MA Chun-guang, HAN Jian-de. Department of Dermatology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 051080, China 【Abstract】 Objective To determine the prevalent herpes simplex virus （HSV） strain in patients with genital herpes （GH）, and to evaluate the sensitivity, specificity, positive predictive value （PPV） and negative predictive value （NPV） of anti-herpes simplex virus type 2 （HSV2） IgG and IgM antibodies in the diagnosis of genital herpes （GH） before in vitro fertilization （IVF）. Methods Totally, 193 HSV2 clinical strains isolated in cell culture from the lesions of patients with GH in the Department of Dermatology, First Affiliated Hospital, Sun Yat-sen University between 2009 and 2011 were typed by using type-specific fluorescein isothiocyanate （FITC）-labelled anti-HSV monoclonal antibodies. Serum samples were obtained from 57 anti-HSV2 IgM/IgG antibody-positive females with suspected GH as well as their husbands （clinical observation group）, 68 HSV culture-positive patients diagnosed with GH （positive control group）, and 120 children aged 8－12 years （negative control group）. Enzyme-linked immunosorbent assay （ELISA） was performed to detect anti-HSV1/HSV2 IgG/IgM antibodies in these serum samples. Statistical analysis was carried out using chi-square test. Results There was a significant difference between the positive control group and negative control group in the positivity rate of anti-HSV1 IgG （89.71% （61/68） vs. 40.80% （49/120）, P < 0.01） and anti-HSV2 IgG （91.18% （62/68） vs. 0, P < 0.01）, but not in that of anti-HSV1 IgM （20.59% （14/68） vs. 21.70% （26/120）, P > 0.05） or anti-HSV2 IgM （13.24% （9/68） vs. 13.30% （16/120）, P > 0.05）. In the clinical observation group, the positivity rate of anti-HSV1 and anti-HSV2 IgM antibodies, anti-HSV1 and anti-HSV2 IgG antibodies was 80.70% （46/57）, 91.23% （52/57）, 84.21% （48/57） and 14.04% （8/57） respectively in the females, 19.30% （11/57）, 8.77% （5/57）, 87.71% （50/57）, 12.28% （7/57）respectively in the males, with significant differences in the positivity rate of anti-HSV1 and -HSV2 IgM antibodies （both P < 0.01）, but not in that of anti-HSV1 or -HSV2 IgG antibodies （both P > 0.05）. The sensitivity, specificity, PPV and NPV were 13.24% （9/68）, 86.67% （104/120）, 36.00% （9/25） and 63.80% （104/163） respectively for anti-HSV2 IgM antibody in the diagnosis of GH, 91.18% （62/68）, 100.00% （120/120）, 100.00% （62/62）, and 95.24% （120/126） respectively for anti-HSV2 IgG antibody. Conclusions HSV2 prevails in the patients with GH in this region, while HSV1 only amounts to 5.18%. The type-specific anti-HSV2 IgG antibody shows a higher specificity, sensitivity, PPV and NPV in the diagnosis of GH than anti-HSV2 IgM antibody, hence, the type-specific anti-HSV2 IgG antibody is superior to anti-HSV2 IgM antibody in diagnosing GH before assisted reproduction. 【Key words】 Herpes genitalis; Simplex virus; Immunoglobulin G; Immunoglobulin M

Key words: Immunoglobulin M