Abstract: SUN Xiu-kun*, TANG Xu, SHEN Hong, YU Jie-kai. *Department of Dermatology, Third People′s Hospital of Hangzhou, Hangzhou 310009, China Corresponding author: SHEN Hong, Email: shenhangzhou@sina.com 【Abstract】 Objective To identify novel biomarkers from urinary protein profiles for the early diagnosis of nephritis in patients with Henoch-Sch?觟nlein purpura by surface enhanced laser desorption/ionization time of flight-mass spectrometry （SELDI-TOF-MS） technique. Methods Urine samples were collected from 60 untreated patients with Henoch-Sch?觟nlein purpura, including 30 patients with nephritis and 30 without. SELDI-TOF-MS technique was used to characterize the protein profile in these urine samples, and the Zhejiang University Cancer Institute - Protein Chip Data Analysis System （ZUCI-PDAS） to identify urine protein markers and construct diagnostic model for nephritis in patients with Henoch-Sch?觟nlein purpura. Results Totally, 154 mass peaks were identified with high quality, and two proteins at a mass-to-charge ratio （m/z） of 2454.971 and 2439.686 showed significantly differential expression between the two groups of patients （P < 0.05）. Seven biomarkers were used to establish a diagnostic model. As estimated by the leave-one-out cross-validation, the diagnostic model distinguished patients with nephritis from those without with a specificity of 71% and sensitivity of 84%. Conclusions The developed diagnostic model based on SELDI-TOF-MS technique and bioinformatics is somewhat specific and sensitive for the prediction of nephritis in patients with Henoch-Sch?觟nlein purpura.