Abstract:

Objective To estimate the effects of rosiglitazone on cultured HaCaT human keratinocytes and their possible mechanism. Methods HaCaT cells were cultured and treated with different concentrations （10, 20, 40, 80 ?滋mol/L） of rosiglitazone or solvent for 24, 48, 72 and 96 hours, respectively. Cell proliferation was detected with methyl thiazolyl tetrazolium （MTT） assay. Western blot was performed to measure the protein expression of β-catenin and cyclin D1. Results Compared with the solvent-treated cells, the proliferation of HaCaT cells was significantly inhibited by 18.9%, 23.7%, 35.1% and 44.6% （all P < 0.05） after treatment with rosiglitazone of 10, 20, 40 and 80 ?滋mol/L, respectively, for 48 hours. The expressions of β-catenin and cyclin D1 were significantly lower in rosiglitazone-treated HaCaT cells than in solvent-treated cells （all P < 0.05）. Conclusion Rosiglitazone could inhibit the proliferation of HaCaT cells, likely by downregulating the expressions of β-catenin and cyclin D1.

Key words: protein, Cyclin D1