Abstract:

Objective To detect and genotype HPV in cutaneous wart tissue. Methods This study recruited 132 outpatients with cutaneous warts in the Affiliated Hospital of Zunyi Medical College. Of the 132 outpatients, 46 had verruca vulgaris, 38 had verruca plantaris, and 48 had verruca plana． Ten healthy human and 15 patients with condyloma acuminatum served as the control. Tissue specimens were obtained from the lesions of these patients as well as from the normal skin of the healthy controls. DNA was extracted from the specimens followed by PCR amplification with general and type-specific primers. Results Of the 132 wart specimens, 128 （97.0%） were positive for HPV, 111（84.1%） for HPV2, 89 （67.4%） for multiple types of HPV, 55 （41.7%） for two types of HPV, 28 （21.2%） for three types of HPV, and 6 （4.6%） for four types of HPV. The most prevalent HPV types were HPV2 followed by HPV1 in verruca vulgaris and verruca plantaris, HPV2 followed by HPV10，HPV3 and HPV1 in verruca plana. Multiple types of HPV were identified in 50.0% （23/46） of the verruca vulgaris specimens，76.3% （29/38） of the verruca plantaris specimens，and 77.1% （37/48） of the verruca plana specimens. Among patients infected with more than one type of HPV, the combination of HPV1 and 2 was the most common （35, 39.3%）, followed the combination of HPV1, 2 and 10 （12, 13.5%）. Conclusions The most prevalent HPV type appears to be HPV2, followed by HPV1, in patients with cutaneous warts in Zunyi area of Guizhou province. HPV2 infection is associated with various types of cutaneous warts. There is a high incidence of coinfection with multiple types of HPV in patients with cutaneous warts, especially in those with verruca plana. Moreover, HPV3 and 10 are newly identified in verruca vulgaris, HPV10 in verruca plantaris，HPV1 and HPV7 in verruca plana.

Key words: HPV genetyping