Chinese Journal of Dermatology ›› 2012, Vol. 45 ›› Issue (3): 173-177.
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Abstract:
Objective To identify differentially expressed proteins in keloid tissue and to explore the proteomic characteristics of keloid by utilizing capillary high-performance liquid chromatography (LC) and tandem mass spectrometry (MS). Methods Tissue specimens were obtained from the lesions of 5 patients with non-familial keloid and normal skin of 5 human controls. After extraction and in-solution digestion of proteins, the resultant peptides were separated and identified by LC-MS/MS. Label-free detection and quantitation of peptides were carried out by using the DeCyder MSTM software. Results A total of 570 differentially expressed peptides were identified between non-familial keloid and normal tissue (all P < 0.05), which correspond to 1221 proteins. A difference greater than 1.5 folds was observed in the expressions of 293 proteins, including 124 up-regulated and 169 down-regulated proteins in non-familial keloid tissue. Conclusion Differences exist in the expression of proteins between non-familial keloid and normal skin tissue.
Key words: Non-familial
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