白念珠菌的药物敏感性与基因型的研究

Abstract

Abstract:

Objective To test the susceptibilities of Candida albicans to 5 antifungal agents including methylrosanilinium chloride and to estimate their relationship with the genotypes of C.albicans. Methods The susceptibilities of 67 C.albicans isolates to methylrosanilinium chloride, fluconazole, amphotericin B, itraconazole and ketoconazole were determined by using a broth dilution method as described in the National Committee for Clinical Laboratory Standards （NCCLS） document M27-A2. DNA was extracted from the C.albicans isolates and subjected to the amplification of 25S ribosomal DNA （rDNA） introns by PCR. The C.albicans isolates were divided into A, B and C genotypes according to the amplicons. The relationship was analyzed statistically by exact probability test between the genotypes and antifungal susceptibility of C.albicans. Results Of the 67 C.albicans isolates, 8.96% were resistant to fluconazole, 2.98% to itraconazole, 1.49% to ketoconazole, and no resistance to amphotericin B was observed. The minimum inhibitory concentration （MIC） of methylrosanilinium chloride varied from 0.125 to 4 mg/L. Statistical analysis revealed no significant correlation between the genotypes and autifungal susceptibilities of C.albicans （all P > 0.05）. Conclusions More attention should be given to the increasing resistance to fluconazole and itraconazole in some isolates of C.albicans as well as to the favorable antifungal activity of methylrosanilinium chloride. The antifungal resistance shows no apparent correlation to the genotypes of C.albicans.

Key words: Genotype

References

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Antifungal susceptibility and genotypes of Candida albicans

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