Abstract:

Objective To explore the effects of natural avocado oil and olive oil on the proliferation and differentiation of HaCaT cells. Methods MTT assay was performed to determine the optimal work concentration of avocado oil and olive oil. Cultured HaCaT cells were divided into 3 groups, i.e., avocado oil group treated with avocado oil of 3% （v/v）, olive oil group treated with olive oil of 3% （v/v）, and control group without any treatment. Immunocytochemistry and immuno-dot-blot method were used to detect the expressions of c-myc, mitogen-activated protein kinase （MAPK）， nuclear factor （NF）-κB, filaggrin, involucrin and keratin10 in HaCaT cells. Results As immunocytochemistry showed, the mean grey values （staining intensity） of c-myc, MAPK, and NF-κB in HaCaT cells were 131.4 ± 6.6，136.3 ± 4.5 and 134.3 ± 5.2 respectively in the avocado oil group, 121.1 ± 4.5, 107.9 ± 7.3 and 106.4 ± 5.4 respectively in the olive oil group, significantly higher than that in the control group （101.9 ± 8.9，91.4 ± 5.1 and 94.3 ± 7.0, respectively, all P < 0.05）, and the avocado oil group was higher than the olive oil group in all the above parameters （all P < 0.05）. Increased expressions of filaggrin, involucrin and keratin 10 were observed in the avocado oil group and olive oil group compared with the control group （all P < 0.05）, and in the olive oil group than in the avocado oil group （all P < 0.05）. The mean grey values of these proteins obtained by immunocytochemisty were significantly correlated with those obtained by immuno-dot-blot method in avocado oil group （r = 0.94, P < 0.01） and olive oil group （r = 0.97, P < 0.01）. Conclusions Certain concentrations of avocado oil and olive oil can promote the proliferation and differentiation of HaCaT cells; avocado oil is more capable to accelerate their growth and proliferation, and olive oil to enhance their differentiation.

Key words: Hacat cell line