Abstract:

Objective To study the effects of 5-aza-dc on the expression of IGFBP7 in and proliferation of melanoma cell lines A375 and M14. Methods Reverse transcription-PCR and immunocytochemistry were performed to detect the mRNA and protein expression of IGFBP7 in A375 cells and M14 cells after treatment with 5-aza-dc of 10 μmol/L for 48 hours, and MTT assay to measure the proliferation of both cell lines treated with 4 different concentrations （2.5, 5, 10, 20 μmol/L） of 5-aza-dc for various durations. Results The treatment with 5-aza-dc restored IGFBP7 expression at both mRNA and protein levels. The four concentrations of 5-aza-dc inhibited the proliferation of A375 and M14 cells in a dose-dependent （F = 561.12, 271.43, respectively, both P < 0.01) and time-dependent （F = 141.35, 549.33, respectively, both P < 0.01） manner. Conclusions DNA methylation may be involved in the modulation of aberrant IGFBP7 gene expression in melanoma, and 5-aza-dc could inhibit the proliferation of A375 and M14 cells.

Key words: 5-aza-dc, IGFBP7, melanoma