实时荧光定量PCR快速鉴定五种常见念珠菌

Abstract

Abstract:

[Abstract] Objective: To establish a Real-time quantitative PCR way in detection and identification of Candida species and explore its feasibility. Method：fungal universal primer ITS4 and ITS86 were designed. Five Candida species were applied to construct recombinant plasmids. Recorded Melting temperature curves after finishing detection by real-time quantitative PCR. Analyzed data. Results: Melting temperatures of five Candida species: (87.33±0.13)℃(Candida albicans), (84.39±0.20)℃(Candida tropicalis), (85.53±0.18)℃(Candida parapsilosis), (86.47±0.10)℃(Candida glabrata), (91.41±0.18)℃(Candida krusei). Conclusion: This study shows the method of Real-time quantitative PCR is a rapid way in detection and identification of Candida species.

Key words: PCR

[1]	. Clinical application of colony PCR in rapid detection of pathogenic fungi causing tinea capitis [J]. Chinese Journal of Dermatology, 2018, 51(6): 434-436.
[2]	. Application of BIOMED-2 primers in the detection of T cell receptor γ gene rearrangements in patients with mycosis fungoides [J]. Chinese Journal of Dermatology, 2013, 46(6): 404-407.
[3]	. Expression of filaggrin in alopecia areata lesions of patients [J]. Chinese Journal of Dermatology, 2012, 45(6): 411-414.
[4]	. Expressions of Toll-like receptor 7 and 9 mRNA in peripheral blood mononuclear cells from patients with vitiligo [J]. Chinese Journal of Dermatology, 2012, 45(4): 249-251.
[5]	Fan YANG. Expressions of Toll-like receptor 3, 7 and 9 in peripheral blood lymphocytes from patients with pityriasis rosea [J]. Chinese Journal of Dermatology, 2012, 45(2): 130-132.
[6]	. Expression of promyelocytic leukemia gene in peripheral blood lymphocytes from patients with psoriasis vulgaris [J]. Chinese Journal of Dermatology, 2012, 45(11): 796-798.
[7]	. Association of HLA-DQA10302, DQB10303 alleles with vitiligo in Uygur nationality in Xinjiang Uygur Autonomous Region [J]. Chinese Journal of Dermatology, 2011, 44(9): 633-635.
[8]	. Expression of FSH1 in Microsporum canis before and after the induction by skin tissue from the scalp and foreskin of children [J]. Chinese Journal of Dermatology, 2011, 44(6): 441-442.
[9]	. Association of HLA-A and -B alleles with syphilis in Shandong Han population [J]. Chinese Journal of Dermatology, 2011, 44(2): 124-126.
[10]	. Primary exploration on identification of pathogenic Trichosporon spp. with rDNA-RFLP analysis [J]. Chinese Journal of Dermatology, 2010, 43(8): 534-537.
[11]	. Rapid detection of pathogenic mycoplasma in genitourinary tract using PCR-reverse dot blot hybridization [J]. Chinese Journal of Dermatology, 2009, 42(12): 814-816.
[12]	LUO Xiao-Yan Li-Ping JIAN G WEI LIU . Relationship between interleukin-18 gene promoter polymorphism and atopic dermatitis in children [J]. Chinese Journal of Dermatology, 2008, 41(7): 447-.
[13]	CHENG Peihua. Quantitation of Genital Herpesvirus DNA with Polymerase Chain Reaction and ELISA [J]. Chinese Journal of Dermatology, 2000, 33(3): 171-172.
[14]	YANG Weiling, WANG Xiaohui, ZHENG Shuyun. Study on the Relationship Between Psoriasis and the Polymorphic Site A677V of Methylenetetrahydrofolate Reductase Gene [J]. Chinese Journal of Dermatology, 1999, 32(2): 87-88.
[15]	Wen Hai, Liao Wanqing, Yao Zhirong. Typing of Cryptococcus neoformans by Random Amplification of DNA Fingerprinting [J]. Chinese Journal of Dermatology, 1998, 31(5): 295-297.

Rapid identification of five common species of Candida with real-time fluorescence-based quantitative PCR

PDF

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 10