Chinese Journal of Dermatology ›› 2009, Vol. 42 ›› Issue (9): 625-627.
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Xiang WenZhongfei wang2,Kang Jian 3
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Abstract: Objective To investigate the role of human interferon (IFN)α-2b in cellular and humoral immune response in BALB/c mice. Methods Plasmids pcDNA3.1(+)-HPV11-E7 and pcDNA3.1(+)- IFNα-2b-linker-HPV11-E7 were constructed. Twenty-eight BALB/c mice were randomly and equally divided into four groups to be immunized with pcDNA3.1(+)-IFNα-2b-linker-HPV11-E7 (group D), pcDNA3.1(+)-HPV11-E7(group C), pcDNA3.1(+)(group B), phosphate buffered solution (group A), respectively, by intramuscular injection. Repetitive immunization was carried out on week 2 and 4. On week 6, mice were killed, splenocytes were isolated, cultured, and stimulated followed by the detection of expression level of IFN-γ, interleukin (IL)-4 and -10 in CD3+ cells and CD4+ cells. Results The level of IFN-γ (expressed as the absorbance at 460 nm) in group C was significantly higher than that in groups A and B (4.36 ± 0.42 vs 3.20 ± 0.41 and 3.29 ± 0.35, both P < 0.001), but lower than that in group D (4.36 ± 0.42 vs 6.39 ± 0.05, P < 0.001), and there was no significant difference between group A and B (P > 0.05). No significant difference was noted in the levels of IL-4 or IL-10 among these 4 groups (P > 0.05). Conclusions IFNα-2b can enhance the immunogenicity of HPV11-E7 and strengthen cellular immunity, especially Th1-type immune response to HPV11-E7 DNA vaccine in mice.
Key words: Condyloma Acuminatum, Human papilloma virus
Xiang WenZhong fei wang Kang Jian. Role of human interferon α-2b as an immunological adjuvant for human papillomavirus (HPV) 11-E7 DNA vaccine[J].Chinese Journal of Dermatology, 2009, 42(9): 625-627.
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