Chinese Journal of Dermatology ›› 2009, Vol. 42 ›› Issue (2): 135-137.
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Objective To explore the susceptibility of Candida albicans biofilms to micafungin. Methods In vitro model of C. albicans biofilm was established in 96-well microtiter plates with 30 C. albicans isolates from the Department of Mycology, Changhai Hospital, Shanghai. The susceptibility of C. albicans biofilms to fluconazole, amphotericine B and micafungin was evaluated by colorimetric XTT-reduction assay. Sessile MIC80 (SMIC80), defined as the lowest antifungal concentration at which an 80% reduction in fungal growth was achieved, was determined. Results Of the 30 C. albicans isolates grown in sessile states, all were resistant to fluconazole (SMIC80≥64 μg/mL), 4 sensitive to amphotericine B (SMIC80≤1 μg/mL), 26 resistant to amphotericine B (SMIC80 > 1 μg/mL), 27 sensitive to micafungin (SMIC80 < 16 μg/mL), 3 resistant to micafungin (SMIC80≥16 μg/mL). Statistical analysis revealed a significant difference in the activity against C. albicans biofilms between micafungin and fluconazole (χ2 = 736.36, P < 0.01), micafungin and amphotericine B (χ2 = 529.95, P < 0.01), but not between anphotericine B and fluconazole (χ2 = 2.29, P > 0.05). Conclusion C. albicans biofilms are resistant to routine antifungal agents such as fluconazole and amphotericine B, but relatively more sensitive to micafungin.
. In vitro activity of micafungin against Candida albicans biofilms[J].Chinese Journal of Dermatology, 2009, 42(2): 135-137.
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