Abstract:

[Abstract] Objective To investigate the influence of Wnt-3a signaling on cultured dermal papilla cell’s growth pattern. Methods Recombinant adenovirus pAdEasy-1/Wnt-3a was constructed at first. After dermal papilla cells were infected by pAdEasy-1/Wnt-3a, growth pattern were observed. By means of laser confocal miscroscopey and Western blot, the expressions of Wnt-3a, β-catenin and Versican were detected. Results Effectively aggregative growth pattern of dermal papilla cells could be induced by over expression of Wnt-3a. After dermal papilla cells were infected by pAdEasy-1/Wnt-3a, laser confocal miscroscopey proved that the fluorescence intensity of β-catenin and Versican expression increased from 34.16±9.62 and 18.81±9.59 to 87.35±17.28 and 92.18±18.39 (β-catenin:t=11.98,p=0.005<0.01; Versican: t=17.88, p=0.000<0.01), while Western blot also proved that β-catenin and Versican expression increased from 15.27±2.17 and 19.32±2.46 to 60.09±7.24 and 58.46±2.78 (β-catenin: t=10.10, p=0.008<0.01; Versican: t=20.63, p=0.000<0.01). Conclusions Wnt-3a signaling is important for dermal papilla cells to retain aggregative growth pattern. Wnt maybe execute this influence though classical pathway. As an important downstream gene of Wnt signaling, Vesicant is an indispensable part for aggregative growth pattern of dermal papilla cell.