Abstract: A 44-year-old woman was admitted to the hospital with a 14-month history of nodules on the dorsum of her left hand that occurred after injury. The following examiniations were performed on the lesional skin: direct microscopic examination, KOH staining and fungal culture. API-20C AUX system was used to identify the carbohydrate assimilation profile. The gene of ribosome DNA was sequenced for the isolates. Random amplification of polymorphic DNA was used to analyse the genotype of the isolates. Histologic sections were subjected to hematoxylin and eosin stain, chloramines silver stain and periodic acid Schiff stain. Antifungal susceptibility of the isolates was analyzed by E-test. The extracellular enzymatic activities were detected by API ZYM system. Hyphae and spores were observed by direct microscopic exami- nation of the lesional tissues. Four times of fungal culture all yielded flat, smooth, cream-colored colonies characteristic of yeast; microscopic examination of the isolates showed clusters of oval spores and short pseudohyphae. The four isolates were consistently identified as Candida guilliermondii according to their morphological characteristics, biochemical test results, and gene sequence of internal transcribed spacer （ITS） and D1/D2 regions of rDNA. All the isolates possessed the same genotype. Histopathological examination showed spores in the stratum corneum as well as a massive infiltration of inflammatory cells and epithelioid granuloma in the dermis. The isolates were resistant to itraconazole with an minimal inhibitory concentration of 1.5 μg/mL. A total of 9 exocellular enzyme activities were detected by API ZYM system. The patient completely recovered after 6 months of oral ketoconazole （0.2 g/d） and topical application of naftifine hydrochloride and ketoconazole cream.