Abstract: Objective To determine the effects of 1320 nm non-ablative laser on the proliferation of human dermal fibroblasts,and the secretion of basic fibroblast growth factor(bFGF)and transforming growth factor-β1(TGF-β1)in vitro.Methods Human dermal fibroblasts were cultured,and irradiated three times by 1320 nm laser at a dose of 15,20 and 24 J/cm²,respectively.The levels of bFGF and TGF-β1 were examined by ELISA at 0,24,48 and 72h after the irradiation.The number of fibroblasts before and after irradiation were determined.Results The number of fibroblasts and the secretion of bFGF both increased after the irradiation at the doses of 20 J/cm² and 24 J/cm²(P<0.05)compared with the control cells.The effect on bFGF secretion of exposure to 20 J/cm² was more significant than that of exposure to 24 J/cm²;the effect peaked at 24h after irradiation.The level of TGF-β1 secretion decreased after irradiation in a dose-dependent manner(15 J/cm² and 20J/cm²,both P<0.05;24 J/cm² P<0.01),and reached a nadir at 24 h.Conclusion The 1320 nm non-ablative laser accelerates the proliferation of fibroblasts,promotes the secretion of bFGF,and inhibits the secretion of TGF-β1 secretion by fibroblasts.

Key words: Fibroblast growth factor 2, Fibroblasts, Laser, Transforming growth factor beta