Abstract: Objective To clone,express,and identify chlamydial GPIC capsid Vp1 gene and protein.Methods The complete sequence of Vp1 gene fromΦCPG1 phage was amplified.The amplicor was cut by restriction endonuclease,linked to plasmid vector,and transformed into E.coli.The expressed protein of recombinant Vp1 was purified and identified.Results The recombinant 1 661 bp gene was sequenced and proved to beΦCPG1 Vp1 by searching Genebank.A 62 kDa capsid protein was expressed and confirmed by SDS-PAGE and Western blot.Conclusion The recombinant Vp1 seems to be a highly conserved and specific marker for chlamydial phage.

Key words: Chlamydiaphage, Vp1 protein, expression