对一株基因工程人抗角蛋白抗体的免疫学鉴定

Abstract

Abstract: Objective To immunologically characterize the binding activity of a genetic engineering human antibody against keratin. Methods The specific anti-keratin Fab was screened from a phage antibody library. After Fab monoclonal antibody was induced by isopropylthio-β-D-galactoside (IPTG), the binding activity with antigens and antigenic specificity of the antibody were verified by ELISA,Western blot, and competitive inhibition ELISA. Results The antibody possessed good antigenic specificity as well as excellent binding activities with antigens, and could recognize 46000 keratin specifically. It was identified as anti-keratin 17 antibody. Conclusions The immunologic characteristics of the genetic engineering antibody are very good. It is necessary to further study the biological activities and clinical application of it.

Key words: Keratin, Antibody, Genetic engineering

LU Ning, WANG Gang, LIU Yu-feng, LI Cheng-xin, LI Wei, ZHANG Hai-long, ZHAO Xiao-dong. Immunologic Characterization of a Genetically Engineered Human Antibody Against Keratin[J].Chinese Journal of Dermatology, 2005, 38(4): 211-213.

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Immunologic Characterization of a Genetically Engineered Human Antibody Against Keratin

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