Abstract: Objective To study the mRNA expression and function of TGF-β1, TGF-βRⅡ and CD105 in the lesional, non-lesional skin of psoriasis and of normal human skin. Methods The RNA of skin tissue was extracted using single-step method of RNA isolation by acid guanidinium thiocyanate. Reverse transcription polymerase chain reaction (RT-PCR) was used to measure the expression of mRNA. Results The mRNA expression of TGF-β1 and TGF-βRⅡ was lower in the epidermis of psoriatic lesion than that of non-lesional psoriatic and normal human skin(P<0.05). There was no significant difference between the normal human epidermis and non-lesional psoriatic epidermis. The mRNA expression of CD105 was higher in the epidermis of psoriatic lesion than that of nonlesional and normal skin(P<0.05). Conclusion The lower mRNA expression of TGF-β1 and TGF-βRⅡ and higher mRNA expression of CD105 in psoriatic lesion may be related to the hyperproliferation of epidermis and dermal inflammatory cell infiltration in psoriasis.

Key words: Psoriasis, Transforming growth factor-beta, Receptors transforming growth factor beta