Chinese Journal of Dermatology ›› 2004, Vol. 37 ›› Issue (2): 88-90.

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Detection of Microsatellite Instability in Lesions from Patients with Coal Arsenic Poisoning

YUAN Wei1, ZHANG Xin-jiang1, WA Qing-biao1, ZHENG Ting-ming1, XIAO He1, DU Hui2, ZHOU Yun-shu2   

  1. Department of Dermatology, Affiliated Hospital, Zunyi Medical College, Zunyi 563003, China
  • Received:2003-02-20 Online:2004-02-15 Published:2004-02-15

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Abstract: Objective In order to elucidate the significance of both microsatellite instability(MSI) and loss of heterozygosity (LOH) in the pathogenesis of arsenic-induced skin lesions, especially skin carcinoma, the presence of MIS and LOH at two loci [chromosome 9p21 (D9S319, p16) and 17p13.1 (TP53.PCR15, p53)] was evaluated. Methods Using polymerase chain reaction(PCR)-denatured polyacrylamide gel electrophoresis-silver staining, MSI and LOH were detected in a total of 34 samples of arsenic-induced skin lesions, and the results were compared with the clinicopathological parameters. Results MSI was found in 32.4% (11/34), and LOH was found in 14.7%(5/34) of 34 samples of arsenic-induced skin lesions. There were no significant differences between the presence of MSI and clinical severity or pathological grades (P>0.05). Conclusions MSI and LOH may play a certain role in the carcinogenesis and progression of arsenic-induced skin lesions.

Key words: Arsenic poisoning, DNA, satellite, Trinucleotide repeat expansion, Loss of heterozy-gosity