副肿瘤性天疱疮伴发Castleman肿瘤分泌致病相关抗体的初步研究

Abstract

Abstract:

Objective To identify features of antibodies in the supernatants of cultured Castleman's disease cells.Methods Lymphocytes of Castleman's disease were isolated and cultured.Immunofluorescence and immunoblot assays were performed with IgG extracted from culture supernatants.The immunoglobulin heavy chaingene of cultured tumor B cells was analyzed by RT-PCR,cloning and sequencing.Results Ig Gextracted from culture supernatant scould attach to the epithelial cell surfaces of mouse bladder tissues.The antibody could also identify two antigen components,210000 and 190000,of normal human epidermal tissues.The sequence similarity was found in immunoglobulin heavy chain gene of cultured tumor Bcells com-pared with that of 6 patients with Castleman's disease previously reported. Conclusions Castleman's tumor associated with paraneoplastic pemphigus can secret autoantibody with similar features to that found in patients'sera.

Key words: Paraneoplastic pemphigus, Sequence analysis

WANG Liang-chun, CHEN Xi-xue, ZHAO Jun-yu, BU Ding-fang, ZHU Xue-jun. A Preliminary Study on the Antibody Secreted in Castleman’s Disease Associated with Paraneoplastic Pemphigus[J].Chinese Journal of Dermatology, 2004, 37(2): 74-76.

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A Preliminary Study on the Antibody Secreted in Castleman’s Disease Associated with Paraneoplastic Pemphigus

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