Abstract: Objective To investigate the effect of a monoclonal anti-interleukin-10 antibody (anti-IL-10 MAb) on systemic candidiasis in a murine model.Methods A cyclophosphamide-induced immunosuppressed murine model of systemic candidiasis was established.Two groups including the control group and the anti-IL-10 MAb treatment group were set in these mice.Colony forming units (cfu) of the kidney and spleen were determined with plating dilution method.The histopathological changes and degree of infection of the liver,spleen and kidney were graded.Meanwhile,the levels of interferon gamma (IFN-Results On the 2nd d,3rd d and 7th d after C. albicans infection the numbers of CFU of the fungi in the kidney in the control group were significantly higher than those in the anti-IL-10 MAb treatment group (P<0.05).The numbers of CFU of the fungi on the 2nd d,3rd d and 7th d after C. albicans infection in the spleen in the control group were also higher than those in the treatment group but without statistically significant difference (P>0.05).The scores of histopathological changes in the kidney in treatment group were lower than those in the control group (P<0.01),and the degree of infection was milder in the treatment group.The scores of histopathological changes in the spleen in the treatment group were also lower than those in the control group but without statistically significant difference (P>0.05).The levels splenic IFN-γ on the 2nd d，3rd d and 7th d alter inleotion in the treatlnent grollp were significantly higher than those in the control group(p<0.01).Conclusion It is demonstrated that the anti-IL-10 MAb has therapeutic effect on murine systemic candidiasis.

Key words: Candidiasis, Animal testing alternatives, Interleukin-10, Antibodies,monoclonal