Abstract: Objective To evaluate the gene mutation of RANTES and CCR5 in SLE and its significance. Methods One hundred and forty-six definitive SLE patients and 159 controls were collected. SNPs of RANTES promoter and polymorphism of CCR5 were performed by PCR or PCR/RFLP assay, and further confirmed by direct DNA sequencing. Results The frequence of RANTES-403G/G compounded with 28C/C and CCR5/CCR5 was significantly different between SLE and control groups (72.6% vs 58.5%, P<0.05, OR=1.88). The frequence of RANTES-403A, -28C haplotype was different between two groups (11.5% vs 16.5%, P<0.05). Frequency of the mutation allele RANTES-403A in renal damage group was lower than that of non-renal damage group and of control(1.49% vs 15.62%, 1.49% vs 17.9%, P<0.05). There was no significantly difference in frequency of the mutation allele RANTES-28C and CCR5Δ32 among renal damage group, non-renal damage group and control (P>0.05). Conclusion These results indicate that the two SNPs are linkage disequilibrium. Interaction of two SNPs in RANTES and CCR5 is related with SLE. RANTES-403G/G compounded with 28C/C and CCR5/CCR5 may be one of risk factors of SLE. RANTES-403A is probably related with renal damage of SLE.

Key words: Polymorphism, single nucleotide, Gene frequency, RANTES, Lupus erythematosus, systemic, Recptors, CCR5