Abstract: Objective To investigate the potential effect of cetyltrimethyl ammonium bromide(CTAB)separated mannan of cell wall from Candida albicans on the production of IL-6 and IL-8 in human peripheral blood mononuclear cells(PBMC)induced by lipopoly saccharide(LPS).Methods PBMCs were pretreated with different concentrations of CTAB mannan(1.000mg/mL、0.100mg/mL、0.010mg/mL)for 24h.LPS(50μg/mL)was added and co-incubated for 24h.And at 48h,the supernatants were collected.At 24h and 48h,only the super-natants of stimulated by CTABmannan were collected.LPS(50μg/mL)was the positive control,unstimulated culture medium the negative control.The contents of IL-6 and IL-8 in the supernatants were determined by ELISA.Results At 24h and 48h,no IL-6 and IL-8 were detected in 3 different concentration-CTAB mannan groups.LPS could induce IL-6(478.507±24.876ng/mL),IL-8(529.655±53.279ng/mL).The contents of IL-6 and IL-8 of negative control were not detectable.In 1.000mg/mL CTAB mannan+LPS group the contents of IL-6were(85.620±16.058ng/mL,P=0.004),IL-8 were(123.940±20.319ng/mL,P=0.011).In 0.100mg/mL CTAB mannan+LPS group,IL-6(210.086±27.874ng/mL,P=0.007),IL-8(206.798±31.878ng/mL,P=0.022).In 0.010mg/mL CTAB mannan+LPS group,IL-6(201.387±32.396ng/mL,P=0.014),IL-8(203.133±36.012ng/mL,P=0.015).Conclusion CTAB mannan of cell wall from Candida albicans could downregulate the production of IL-6 and IL-8 from human peripheral blood mononuclear cells induced by LPS.

Key words: Candida albicans, Mannans, Interleukin-6, Interleu kin-8