Chinese Journal of Dermatology ›› 2001, Vol. 34 ›› Issue (5): 352-354.

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Molecular Determination of Dermato phyte Fungi Using Arbitrarily Prime d Polymerase Chain Reaction

KONG Xiangming1, YANG Guoling1, JIN Li ji2   

  1. Department of Dermatology, First Affiliated Hospital, Dalian Medical University, Dalian 116011, China
  • Received:2000-11-06 Online:2001-10-15 Published:2001-10-15

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Abstract: Objective To study the significance of AP-PCR in identification and subtyping of dermatophytes.Methods Using a pair of random primers,OPAA11(5'-ACCCGACCTG-3'),and OPD18(5'-GAGAGCCAAC-3') the DNAs of 64 isolates of dermatophytes(9 species of 3 genera),Sporothrix schenckii and Candida albicans were amplified by AP-PCR and analysed by electrophoresis.Results Distinct DNA band patterns were observed in different dermatophyte species.Commonmajor DNA bands were observed in Trichophyton rubrum isolated from different areas with strain difference.Conclusion Using OPAA11 and OPD18 as primers,AP-PCR may be applied in the identification and subtyping of dermatophytes.

Key words: Dermatophyte, Arbitrarily primed p olymerase chain reaction