Abstract:

Objective To investigate the role of allele loss on chromosome 9 in the pathogenesis of basal cell carcinoma, squamous cell carcinoma and Bowen's disease. Methods Two polymorphic microsatellite sequences at loci D9S319 (9p21) and D9S299 (9q22.2-q22.3) were examined by polymerase chain reaction for loss of heterozygosity. Results Loss of heterozygosity with D9S319 (9p21) marker was not observed in 10 informative cases of sporadic basal cell carcinoma, 19 cases of squamous cell carcinoma and 4 cases of Bowen's disease. Allelic deletion of D9S299 was not found in all 21 informative cases of squamous cell carcinoma and 4 cases of Bowen's disease. D9S299 (9q22.2-22.3) allele loss occurred in 2 of 10 informative cases of sporadic basal cell carcinoma, indicating that there might be a susceptible tumor suppressor gene on chromosome 9. Conclusion Our findings suggest that chromosome 9q22.2-22.3 might contain a putative tumor suppressor gene, and loss of which may play an important role in the pathogenesis of basal cell carcinoma.

Key words: Carcinoma basal cell, Carcinoma,squamous cell, Bowen’s diseas, Loss of heterozygosity, Chromosomes,human,pair 9