Abstract: Objective To report a rare case of prolidase deficiency and the methods of diagnosis and treatment. Methods The activity of prolidase (PD) in serum and skin fibroblasts was detected by high-performance liquid chromatography (HPLC) or by colorimetric determination, and the level of glycine L-proline in urine was tested. The skin ulcer was topically treated with an emulsion containing 5% proline and 5% glycine (EPG). Results The activity of PD in the patient's serum, urine and fibroblasts was abnormal, and topical EPG reduced the size of ulcer. Conclusion HPLC and colorimetric determination are useful diagnostic tools, and the topical EPG therapy is helpful.

Key words: Prolidase, Fibroblasts