Abstract: Objective To set up serotyping methods of Ureaplasma urealyticum by molecular biological techniques.Methods Primers for typing were designed and different serotypes of Ureaplasma urealyticum were identified by PCR and restriction endonuclease digestion.Results PCR primers UMS51, UMAU1 and UMS51,UMAU3 was specific to Uu1,Uu6 and Uu3, Uu14 respecitively. To distinguish Uu1 and Uu6, restriction endonuclease digestion were used.Conclusion By PCR and restriction endonuclease digestion on the basis of DNA sequence of N terminal of multiple-banded antigen gene, serovars of biovar one of Uu can be distinguished but Uu3 and Uu14 can not be distinguished.

Key words: Ureaplasma urealyticum, Serotyping