Abstract: Objective and Methods To determine the inhibitory activity of dipyridamole (DPM) on replication of herpes simplex virus (HSV)-Ⅰ、Ⅱ in Vero cells and HEK-293 cells in vitro. Results The results showed that the range of 50% inhibition concentrations (IC₅₀) of DPM and control drug acyclovir on different types of HSV in 2 cell lines ranged 6.9～16.1μg/ml and 1.9～21.3μg/ml respectively. Conclusion It is known that DPM possessed a definite antiviral activity with a broad spectrum, the therapeutic effect of DPM taken orally for the treatment of viral diseases should be further studied. It is possible that topical preparations of DPM can be used for the treatment of mucocutaneous infection of HSV.

Key words: Dipyridamole, Herpesvirus 1,human, Herpesvirus 2,human, Antiviral agents