Abstract: Objective To elucidate the role of signal transduction TNF receptor I(TNF R1) and TNF receptor (TNF R2) in Langerhans cells (LC) migration in immuno-responses,TNF R1gene-knock-out mutant mice were used in this study.Methods The LC migration from skin to regional lymph nodes after FITC sensitizatioon in both the TNF R1-deficient mice and the TNF R1-deficient mice pretreated with neutralizing antibody to TNFα were examined by im munolabelling and FCM,and TNFR1⁺/+ mice were used as control. Results Following FITC sensitization there was no significant difference between the number of FITC⁺ cells(LC) found in lym ph nodes from TNF R1-deficient mice,and from the wild type (TNFR1⁺/+) mice,and the number of LC found in the draining lymph nodes fromanti-TNFα antibody pretreatment TNF R1-deficient mice was significantly lower than that of the mice not pretreated by anti-TNFα antibody. Conclusion It suggests that TNFR1is not necessary in mediating LC migration and TN-F R2 might be crucial in signal transduction in TNF R1-deficient mice.

Key words: ReceptorⅡ, Tumor necrosis factor, Langerhans cells, Signal transduction