Chinese Journal of Dermatology ›› 1997, Vol. 30 ›› Issue (3): 157-159.
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Yang Huilan1, Li Ming2, Liao Yuanxing1
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Abstract: Herpes simplex virus glycoprotien D gene was amplified from HSV-2 Sav strain. The D gene fragment was cloned into M13mp18 and M13mp19 and sequenced.The gD and expression vector(PWR450-1) were recombined, the recombinants could express high level corresponding beta galactosidase antigen fusion proteins in E.coli.The SDS-PAGE analysis demonstrated that the expressed fusion protein consists of 50.1% of total bacterial proteins. The immunogenicity of the product was tested with dot-ELISA and Western blot. Preliminary purification of gD galactosidase fusion protein showed that the product exhibied a purity over 90%.The results of investigation will help to study the immunogenicity of gD and produce genetic engineering vaccine of genital herpes simplex.
Key words: Herpes simplex virus, Glycoprotein D
Yang Huilan, Li Ming, Liao Yuanxing. High Level Expression of Glycoprotein D of Herpes Simplex Virus in Escherichia Coli[J].Chinese Journal of Dermatology, 1997, 30(3): 157-159.
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