Chinese Journal of Dermatology ›› 1996, Vol. 29 ›› Issue (3): 175-177.

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Comparison of Polymerase Chain Reaction and Cell Culture for Detection of Chlamydia Trachomatis in Urogenital Specimens

Zhang Shuwen, Wang Heying, Ye Shunzhang   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences, Peking Union Medical Colege, Nanjing 210042
  • Received:1995-07-04 Revised:1995-10-12 Online:1996-06-15 Published:1996-06-15

Abstract: A study to compare the polymerase chain reaction(PCR) test with the cell culture method in detection of Chlamydia trachomatis in urogenital specimens infections was performed.Urogenital specimens were simply lysed by nonionic detergents for 10 minutes and subsequently subjected to amplification without prior extraction of nucleic acid.

Key words: Chlamydia trachomatis, Polymerase chain reaction(PCR), Cels,cultured