Chinese Journal of Dermatology ›› 1996, Vol. 29 ›› Issue (3): 157-160.

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The Kinetics of Cytokine Gene Expression in PBMC from HIV Seropositive Subjects Stimulated by Phy-tohaemagglutinin,Pokeweed Mitogen,Candidin,Anti-CD 3 Monoclonal Antibody and Recombinant IL-2

Fan Jiang1, Chen Ruoyian1, Thang Nguyen2   

  1. 1. Institute of Dermatology, Chinese Academy of Medical Sciences, Peking Union Medical Colege, Nanjing 210042;
    2. UCLA, School of Medicine, Los Angeles, USA
  • Received:1995-02-20 Revised:1995-07-13 Online:1996-06-15 Published:1996-06-15

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Abstract: Cytokines were closely related to the major immunoregulatory mechanism in infectious disease.The kinetics of cytokine gene expressions including IL-2,IFN-γ,IL-10,IL-6,TNF-α and IL-2 receptor in PBMC from HIV seropositive and seronegative subjects were compared by quantitative reverse transcription polymerase chain reaction studies stimulated by phytohaemaglutinin(PHA),pokewed mitogen(PWM),candidin,anti-CD3 monoclonal antibody (Anti-CD3m Ab) and recombinant IL-2(rIL-2).IL-2 and IL-2 recept or (IL-2R) to PHA,PWM,candidin,anti-CD3 mAb and rIL-2 stimulation were markedly lowered in patients with HIV infection.

Key words: HIV, Cytokine, Stimulation