Abstract: In this paper, organisms in biopsy specimens of 82 cases of sporotrichosis were studied.The results showed that in the histopathological sections with HE stain asteroid bodies were observed in 22/82 (26.8%) sections, spores in 36/82 (43.9%) sections.of 45 sections with PAS stain asteroid bodies were found in 9 (20%) sections,spores in 38(84.4%),and mycelia in 2.Spores Were found in all of 5 cases with multi-organisms under TEM, one spore was being wrapped by the pseudopodium of a neutrophil and going to be phagocytosed.Spores were found in all of 4 cases with fluorescent antibody staining.In smears exudates from lesion, spores were found in 40 cases (88.8%) and asteroid bodies in 1 case (2.2%) with Gram and PAS stain in 45 cases.Spores with different shapes were found in 5 cases under SEM,and in all of 16 cases with fluorescent antibody stainig.The pathological tissues of 52 cases were cultured, and 47 cases (90%) were confirmed to be Sporothrix schenkii.Skin test of sporotrichin were performed in 38 cases,positive results were observed in all cases.

Key words: Sporotrichosis, Sporotrichum