Abstract: Thirteen Lesions were excised from 13 patients with condyloma acuminatum (CA).Thirteen lesions taken from patients with basal cell carcinoma (BCC) were studied as controls.Epidermis was separated from dermis by treatment with sodium bromide.DNA extracted from both tissues and sodium bormide solution were amplified respectively for the presence of HPV DNA using the polymerase chain reaction.HPV DNA was detected in the epidermis of 11 specimens of CA,HPV type 6 in seven and HPV type 11 in four.HPV DNA was found in the dermis of three specimens of CA;type 6 in two and type 11 in one.Two specimens were excluded because of contamination of the sodium bromide solution by HPV' HPV DNA was not detected in the lesions from CA patients.The presence of HPV DNA in the dermis of some lesions may explain the recurrence of the disease in sporadic cases.

Key words: Condyloma acuminatum, Human papilloma virus, Polymerase chain reaction