Abstract: Between June 1990 and June 1991,we examined 836 specimens from patients attending gynaecological out-patient clinic and sexually transmissible disease clinic of some hospitals in Guangdong.The specimens were inoculated in eycloheximide-treated MeGay cells at 37℃,5% CO₂ for 48 hours and then the chlamydia trachomatis antigen ivcre detected by fluorescence labeled monoclonal antibodies(Micro Trak Syva).The positive rate far chlamydia trachomatis from female specimens(endocervical swabs) was 8.4%(53/631),the positive rate for male specimens(uragenital swabs) was 14.6%(30/205).We used a 24-well tissue culture plate for thr isolation of chlamydia trachomatis instead of flat-hottom vials containing a cover slip.The positive rate of immunafiuorescence staining is 2-3 times higher than that of iodine staining,the difference is very significant(P<0.005).

Key words: Chlamydia, trachomatis, Chlamydial infections