Abstract: An attempt is made to produce DNA probe that only hybridizes with the DNA from Candida albicans.Total DNr1 was iaolated from the cells of Candida albicans and digested with EcoRI restriction enzyme.A fragmont(2.6 Kb) was cloned into the EcoRI site of the plasmid vector pBR322 and replicated in E.coli K802.The candidal fragment was labelled with digoxijenin-dUTP(DigdUTP) by random primed DNA labelling method and used to probe dot blots of DNA from several Candida spacious and human leukocytes.Only the DNA from Candida albicans and a strain of Candida steliatoidea was found to contain sedtrences of significant homology for hybridization.From tlue above results,it is concluded that this cloned fragment may passibly be used as a DNA probe for detecting and identifying the presence of Candida albicans.

Key words: Candida albicans, DNA probes, Molecular coning DigoxigenindUTP, DAN labeling