Abstract: This article reports the results of in vitro antifungal activity of home-made ciclopirox olamine. Among 12 strains of common pathogenic fungi, the lowest MIC of the drug was 1.95μg/ml, the highest 31.3μg/ml, and the other 10 ones ranged from 3.9~7.8 μg/ml. The MIC values of staphylococcus aureus and Escherichia coli were 31.3μg/ml. The therapeutic effect of 1% ciclopirox olamine and 2% miconazole nitrate creams was compared in self-control of 29 guinea pigs experimentally infected with T. mentagrophyte.