Chinese Journal of Dermatology ›› 2021, Vol. 54 ›› Issue (3): 220-225.doi: 10.35541/cjd.20200743

• Original Articles • Previous Articles     Next Articles

Effect of ribosomal protein L34 gene knockdown on a human cutaneous squamous cell carcinoma cell line SCL-1

Zhang Xueli, Guo Yan, Su Minjing, Liu Yu, Huang Yanping, Li Xin, Sun Zhiqiang, Han Jianwen   

  1. Department of Dermatology and Venereology, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010010, China
  • Received:2020-07-24 Revised:2021-01-06 Online:2021-03-15 Published:2021-03-02
  • Contact: Han Jianwen E-mail:hanjianwen1981@hotmail.com
  • Supported by:
    National Natural Science Foundation of China（81660513）; Natural Science Foundation of Inner Mongolia Autonomous Region（2018MS08030）; Science and Technology Planning Project of Inner Mongolia Autonomous Region （201503004, 2019GG082）; The Young Innovative Talents Training Project of "Prairie Talents" of Inner Mongolia Autonomous Region; CAS "Light of West China" Program

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Abstract: 【Abstract】Objective To investigate the effects of knockdown of the RPL34 gene on the proliferation and apoptosis of human cSCC cells. Methods First, RPL34 expression in the skin tissues of 14 patients with cSCC and 16 normal control subjects was analyzed using immunohistochemistry. Next, siRNA-lentivirus was used to knockdown RPL34 expression in the cSCC cells SCL-1. PI-FACS was used to measure cell cycle distribution and apoptosis, and MTT assay was also performed,the knockdown efficiency of target gene was detected by qPCR, and the expression level of RPL 34 was analyzed by Western blot. Chi-square test was used to compare the counting data, and the mean ± standard deviation was used to express the measurement data. When α was set to 0.05, P < 0.05 was considered to be statistically different. Results In immunohistochemistry, the expression of RPL 34 in cSCC tumor tissue was significantly higher than that in normal skin tissue (the score of nuclea expression in cSCC group was 2.929±1.542，the score of cytoplasm expression in cSCC group was 2.143±1.956, that in normal skin tissue was 2.563 ± 1.153, and that in cytoplasm was 0.500 ± 0.516, P < 0.05). The apoptosis rate was 4.58% in blank control group and 9.42% in RPL 34 shRNA group (P < 0.05). Cell cycle distribution in the experimental group (shRPL 34) increased in S phase (P < 0.05), decreased in G1 phase (P < 0.05), and no significant change was found in G2 / M phase (P > 0.05). MTT assay showed that the absorbance and absorbance of SCL-1 cells CD490 in shRPL 34 group were significantly lower than those in blank control group (P < 0.05), indicating that the number of viable cells in shRPL34group was significantly lower than that in control group. In qPCR results, the expression of RPL 34 gene in SCL-1 cells was inhibited by shRNA lentivirus infection, and the knockdown efficiency was 85.1% (P < 0.05). Conclusion RPL34 can regulate cell proliferation and apoptosis, as well as interfere with the cell cycle, in cSCC.

Key words: Carcinoma, squamous cell, Ribosomal proteins, RNA, small interfering, Cell cycle, Apoptosis, RPL34, SCL-1 cells