Chinese Journal of Dermatology ›› 2021, Vol. 54 ›› Issue (4): 364-367.doi: 10.35541/cjd.20190964

• Reviews • Previous Articles    

Detection methods for allergen-specific T cells

Xu Beilei, Yao Xu   

  1. Department of Allergy and Rheumatology, Hospital of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042, China
  • Received:2019-10-08 Revised:2020-04-25 Online:2021-04-15 Published:2021-03-31
  • Contact: Yao Xu E-mail:dryao_xu@126.com
  • Supported by:
    National Natural Science Foundation of China(81371735, 81573045); Natural Science Foundation of Jiangsu Province (BK20150166); CAMS Innovation Fund for Medical Sciences (2016-I2M-1-005)

Abstract: 【Abstract】 Tracking changes in the number and function of T cells is of great value to clinical diagnosis and evaluation of the efficacy of allergen immunotherapy. This review summarizes research progress in detection methods for allergen-specific T cells and their application, such as carboxyfluorescein succinimidyl ester dilution assay, enzyme-linked immunospot assay, intracellular cytokine staining assay and microarray immunosensors, providing references for selecting and developing appropriate detection methods in clinical practice.

Key words: T-cell antigen receptor specificity, Major histocompatibility complex, Enzyme-linked immunospot assay, Allergic diseases, Allergen-specific T cells, Carboxyfluorescein succinimidyl ester dilution assay, Intracellular cytokine staining assay, Microarray immunosensors