中华皮肤科杂志

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慢性荨麻疹患者外周血单一核细胞CC型趋化因子的表达

唐慧, 徐金华, 郑志忠   

  1. 复旦大学附属华山医院皮肤科 200040
  • 收稿日期:2005-07-01 出版日期:2006-02-15 发布日期:2006-02-15

The mRNA expression of CC chemokines in peripheral blood mononuclear cells from patients with chronic urticaria

TANG Hui, XU Jin-hua, ZHENG Zhi-zhong   

  1. Department of Dermatology, Huashan Hospital, Fudan University, Shanghai 200040, China
  • Received:2005-07-01 Online:2006-02-15 Published:2006-02-15

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摘要： 目的 研究CC型趋化因子家族中调节激活正常T细胞表达和分泌的细胞因子(RANTES)、单核细胞趋化蛋白-1(MCP-1)、单核细胞趋化蛋白-3(MCP-3)和巨噬细胞炎症蛋白(MIP-1α)的mRNA在慢性荨麻疹患者外周血单一核细胞中的表达情况及其相互关系;探讨CC型趋化因子的水平与慢性荨麻疹患者血清总IgE和嗜酸粒细胞阳离子蛋白水平的相关性.方法 逆转录聚合酶链反应(RT-PCR)方法检测31例慢性荨麻疹患者和22例正常人对照外周血单一核细胞中RANTES、MCP-1、MCP-3和MIP-1α的mRNA的表达水平.结果 ①与正常人对照相比,慢性荨麻疹患者外周血单一核细胞中RANTES、MCP-1、MCP-3和MIP-1α的mRNA表达水平均明显增高(P<0.001).②慢性荨麻疹患者中RANTESmRNA的表达水平与MCP-1、MCP-3、MIP-1α的水平呈正相关(P<0.05),MCP-1与MIP-1α的mRNA表达水平之间亦呈正相关(P<0.01),但MCP-1与MCP-3之间、MCP-3与MIP-1α之间的mRNA表达水平无显著相关(P>0.05).③慢性荨麻疹患者中RANTES的mRNA表达水平与血清总IgE和嗜酸粒细胞阳离子蛋白的水平呈负相关(P<0.05);MCP-1、MCP-3和MIP-1α的mRNA表达水平与血清总IgE和嗜酸粒细胞阳离子蛋白的水平之间无显著相关(P>0.05).结论 慢性荨麻疹患者外周血单一核细胞中RANTES、MCP-1、MCP-3和MIP-1α的mRNA表达水平的上调可能与荨麻疹的发病有关.

关键词: 趋化因子,CC, 白细胞,单核, 荨麻疹

Abstract: Objective To investigate the mRNA expression of CC Chemokines,including RANTES (regulated upon activation,normal T cell expressed and secreted),monocyte chemoattracctant protein-1 (MCP-1),monocyte chemoattracctant protein-3 (MCP-3) and macrophage inhibitory protein 1α (MIP-1α),in peripheral blood mononuclear cells (PBMCs) of patients with chronic urticaria,their mutual correlation,and the relationship between the expression level of CC chemokines and the serum level of IgE and eosinophil cationic protein (ECP) in the patients.Methods Reverse transcription-polymerase reaction (RT-PCR) was applied to semiquantitatively analyze the mRNA expression of RANTES,MCP-1,MCP-3 and MIP-1α in PBMCs from 31 patients with chronic urticaria and 22 healthy subjects.Results The mRNA expression of RANTES,MCP-1,MCP-3 and MIP-1αwas significantly higher in the patients than those in the normal controls (P<0.001).There was a positive correlation (P<0.05) between the mRNA expression of RANTES and that of MCP-1,MCP-3 and MIP-1α in the patients;the mRNA expression of MCP-1 positively correlated (P<0.01) with that of MIP-1α,while there was no correlation between the mRNA expression of MCP-1 and MCP-3,or between that of MCP-3 and MIP-1α(P >0.05).In the patients,the mRNA expression of RANTES negatively correlated with the serum level of IgE and ECP (P<0.05).Conclusions The results suggest that the up-regulation of the mRNA expression of RANTES,MCP-1,MCP-3 and MIP-1α might play a role in the pathogenesis of chronic urticaria.

Key words: Urticaria, Chemokines,CC, Leukocytes,mononuclear