中华皮肤科杂志

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内皮缩血管肽1和促肾上腺皮质激素对体外培养的正常人黑素细胞增殖的影响

杜娟1, 徐前喜1, 何培英2, 张建中1, 朱铁君1   

  1. 1. 北京大学人民医院皮肤科 100044;
    2. 北京大学人民医院中心实验室 100044
  • 收稿日期:2005-04-30 出版日期:2006-02-15 发布日期:2006-02-15
  • 基金资助:
    教育部教育振兴行动计划特殊专向(985工程)基金资助项目

The effects of endothelin-1 and ACTH on the prolifertation of cultured normal human melanocytes

DU Juan1, XU Qian-xi1, HE Pei-ying2, ZHANG Jian-zhong1, ZHU Tie-jun1   

  1. Department of Dermatology, People's Hospital, Peking University, Beijing 100044, China
  • Received:2005-04-30 Online:2006-02-15 Published:2006-02-15

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摘要： 目的 了解内皮缩血管肽1及促肾上腺皮质激素(ACTH)对体外培养的正常人黑素细胞增殖的影响.方法 采用体外黑素细胞培养技术、四甲基偶氮唑蓝比色法(MTT)研究不同浓度内皮缩血管肽1、ACTH对黑素细胞增殖的影响.结果 从0.1~1000nmol/L浓度的内皮缩血管肽1均可不同程度地促进正常人黑素细胞的增殖,低浓度0.1nmol/L内皮缩血管肽1具有很好的促增殖作用.10-13~10-9mol/L的ACTH也可不同程度地促进正常人黑素细胞的增殖,与对照组相比,P值均<0.05,其中又以高浓度10-9mol/LACTH促增殖能力最强,10-12mol/L、10-11mol/L、10-10mol/L 3个浓度间差异无统计学意义.结论 内皮缩血管肽1及ACTH具有促进体外培养的正常人黑素细胞增殖的作用,较低浓度内皮缩血管肽1即具有很好的促增殖作用,正常人生理水平(10-12~10-11mol/L)及更高浓度ACTH均有较强的促增殖作用.

关键词: 黑素细胞, 内皮缩血管肽1, 促肾上腺皮质激素

Abstract: Objective To study the effects of endothelin-1 (ET-1) and adrenocorticotropic hormone (ACTH) on the proliferation of cultured normal human melanocytes.Methods Normal human melanocytes were obtained from the foreskins of healthy men,and cultured in minimum essential medium (MEM) with 10% bovine serum.The cultured melanocytes of the 3rd passage were treated with various concentrations of ET-1 and ACTH.Cell proliferation was assessed by the MTT method.Results ET-1 (0.1~1 000 nmol/L) promoted the proliferation of cultured human melanocytes.ACTH (10-13~10-9 mol/L) also promoted the proliferation of cultured human melanocytes (P<0.05),with 10-9 mol/L being the most efficient concentration.There was no significant difference in the effect of ACTH at the concentrations of 10-12 mol/L,10-11 mol/L and 10-10 mol/L.Conclusions ET-1 and ACTH could promote the proliferation of melanocytes,which occurs even at low concentrations of ET-1.ACTH,at physiological (10-12 and 10-11 mol/L) or higher concentrations could also promote the proliferation of melanocytes.Therefore,these two factors might be utilized in non-carcinogen culture and transplantation of melanocytes as well as direct treatment of vitiligo in the future.

Key words: Melanocyte, Endothelin-1, Corticotropin