中华皮肤科杂志 ›› 2013, Vol. 46 ›› Issue (2): 97-99.

• 论著 • 上一篇    下一篇

辣椒和乙醇对肉桂醛引起的兔耳刺激性接触性皮炎的影响

李周娜1,金哲虎2   

  1. 1. 延边大学附属医院皮肤科
    2. 吉林省延吉 延边大学附属医院皮肤科
  • 收稿日期:2012-03-12 修回日期:2012-08-20 出版日期:2013-02-15 发布日期:2013-02-01
  • 通讯作者: 金哲虎 E-mail:jinzh_621@163.com

Influence of pepper and alcohol on irritant contact dermatitis induced by cinnamaldehyde in rabbit ears

Ju Na-Lee LEEJu Na1,   

  • Received:2012-03-12 Revised:2012-08-20 Online:2013-02-15 Published:2013-02-01

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摘要： 目的 探讨辣椒和乙醇对肉桂醛引起的皮肤刺激性接触性皮炎的影响。方法 日本大耳白兔32只，随机分成对照组、乙醇组、辣椒组、混合组，每组8只。对照组：生理氯化钠溶液日2次灌胃；乙醇组：50%乙醇、生理氯化钠溶液分别灌胃；辣椒组：10%辣椒液、生理氯化钠溶液分别灌胃；混合组：50%乙醇、10%辣椒液分别灌胃。剂量均为每次8 ml/kg，各组连续灌注7 d。出现饮水量增加、大便干结、耳廓血管充血等辛辣刺激体征后，再将各组实验动物分成完整皮肤组和破损皮肤组，每组4只，按化妆品卫生规范进行皮肤刺激性实验，分别记录1、24、48和72 h刺激部位的情况，对皮肤刺激反应进行评分，组间差异比较采用方差分析。结果　辣椒与乙醇连续7 d灌胃后，对照组症状体征评分为0.25 ± 0.46，辣椒组为5.38 ± 0.74，乙醇组为7.25 ± 0.71，混合组为12.75 ± 0.70，辣椒组、乙醇组及混合组的症状体征评分明显高于对照组，差异有统计学意义（P < 0.01）。在家兔完整皮肤刺激状态评分中，混合组皮肤刺激反应强度在24、48 h显著高于灌注辣椒组（F = 28.44、30.33，P < 0.05）；在家兔破损皮肤刺激状态评分中，乙醇组、混合组皮肤刺激反应强度在24、48、72 h评分显著高于辣椒组（F = 197.12、94.54、87.63，P < 0.01）。结论 按照实验所用的剂量，辣椒、乙醇和混合物都能够在家兔诱导出辛辣刺激体征，辣椒和乙醇能明显增加皮肤对肉桂醛刺激的敏感性。

关键词: 皮炎，刺激性, 辣椒属, 肉桂醛, 乙醇, 疾病模型，动物

Abstract: LI Zhou-na， JIN Zhe-hu. Department of Dermatology, Affiliated Hospital of Yanbian University, Yanji 133000, Jilin, China Corresponding author: JIN Zhe-hu, Email: jinzh_621@163.com 【Abstract】 Objective To estimate the influence of pepper and alcohol on irritant contact dermatitis induced by cinnamaldehyde in rabbit ears. Methods Thirty-two Japanese large-ear white rabbits were used in this study, and equally divided into 4 groups to receive intragastric infusion of sodium chloride physiological solution twice daily （control group）, sodium chloride physiological solution once and 50% alcohol solution once daily （alcohol group）, 10% pepper solution once and sodium chloride physiological solution once daily （pepper group）, 50% alcohol solution once and 10% pepper solution once daily （mixture group）, for 7 days. The dose of these solutions for intragastric infusion was uniformly 8 ml/kg. After the appearance of symptoms of irritant contact dermatitis （including increase in water intake, dry stool, vasocongestion of auricle of ears）, the back skin of 4 mice in each group was injured and served as the injured skin subgroup, and the back skin of the other 4 mice in each group remained uninjured and served as the intact skin subgroup. Then, skin irritation test was carried out according to the Hygienic Standard for Cosmetics on both sides of the back of these rabbits with 2% cinnamaldehyde （irritant area） and 70% alcohol （control area） respectively. Skin reaction at the irritated sites was observed and scored at 1, 24, 48 and 72 hours after the irritation. Analysis of variance was conducted to assess the differences in reaction intensity between these groups. Results After 7 days of intragastric infusion, the symptom score was 0.25 ± 0.46 in the control group, significantly lower than that in the pepper group （5.38 ± 0.74, P < 0.01）, alcohol group （7.25 ± 0.71, P < 0.01） and mixture group （12.75 ± 0.70, P < 0.01）. In rabbits with intact skin， the intensity of irritant skin reaction was significantly stronger in the mixture group than in the pepper group at 24 and 48 hours （F = 28.44, 30.33, respectively, both P < 0.05）, while in rabbits with injured skin，the irritant skin reaction was more intense in the alcohol group and mixture group than in the pepper group at 24, 48 and 72 hours（F = 197.12, 94.54, 87.63, respectively, all P < 0.01）. Conclusions Pepper and alcohol alone or in combination at the tested concentration can induce irritation symptoms in rabbits, and both of them can enhance the response of skin to irratation by cinnamaldehyde. 【Key words】 Dermatitis, irritant; Capsicum; Ethanol; Cinnamaldehyde; Disease models, animal

Key words: cinnamic aldehyde