中华皮肤科杂志 ›› 1994, Vol. 27 ›› Issue (2): 67-69,126.

• 论著 •    下一篇

M-Dot-ELISA检测麻风家内接触者血清中麻风杆菌特异性抗原

曹元华1, 和泉真藏2, 吴勤学1, 叶干运1, 川津邦雄2, M. D. Amiruddin3, Mauro F. Mendes4, 侯伟1   

  1. 1. 中国医学科学院、中国协和医科大学皮肤病研究所, 南京, 210042;
    2. 日本厚生省国立多摩研究所;
    3. 印度尼西亚Hasanuddin大学;
    4. 巴西Sociedde Filaatropical Humanitas
  • 收稿日期:1993-03-05 修回日期:1993-04-19 出版日期:1994-04-15 发布日期:1994-04-15

Detection of M. leprae-specific Antigen with M-Dot-ELISA in Sera from Household Contacts of Leprosy Patients

CAO Yuan-Hua1, IZUMI Shinzo2, WU Qin-Xue1   

  1. Dermatology, Chinese Academy of Medical Sciences, Peking Union Medical College, Nanjing 210042
  • Received:1993-03-05 Revised:1993-04-19 Online:1994-04-15 Published:1994-04-15

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摘要： 用改良酶联免疫斑点15试验(简称M-Dot-ELISA)对麻风家内接触者(HC)血清进行了麻风杆菌特异性酚酚糖脂I(PGL-I)抗原检测。75例HC均经麻风杆菌明胶微粒凝集试验(MLPA)和酶联免疫吸附试验(ELISA)证实为麻风杆菌特异性抗体阳性者。结果表明:①75例HC中抗原阳性者16例,阳性率为21%.接触多菌型麻风(MB)者的抗原阳性率(28%)明显高于接触少菌型麻风(PB)者(0%),两者之间有极显着性差异(P<0.01).②抗体弱、强阳性组的抗原阳性率和抗原量之间亦有极显着差异(P<0.01).③40例正常人及10例经MLPA和ELISA检测证实特异性抗体阳性的非麻风患者血清标本,抗原检测均阴性。

关键词: 麻风, 接触者, 抗原, 细菌

Abstract: We report the results of detecting M.lepraespecific antigen-phenolic glycolipid I (PGL-I) with modified M-Dot-ELISA in sera from 75 cases of household contacts (HC)of leprosy patients, which were all seropositive by Gelatin Particle Agglutination Test (MLPA)and ELISA.The results indicate that:(1) 16/75 (21.3%) are antigen positive.The rates of positivity in HC of MB patients are much higher than those in HC of PB patients,the difference (P<0.01) between them is significant.(2) Positive rates and levels of PGL-I in HCs are associated with levels of antibody against PGL-I in sera from HCs.There are significant differences in antigen positive rates and levels between weak and strong antibody positive groups of HC (P<0.01).(3)The sera from 40 normal controls and 10 nonleprosy patients with positive anti-PGL-I antibody by MLPA and ELISA are antigen negative.

Key words: Leprosy, Contacts, Antigens, bacterial