中华皮肤科杂志 ›› 1996, Vol. 29 ›› Issue (1): 35-37.

• 论著 • 上一篇    下一篇

不同方法制备类天疱疮抗原的比较研究

林麟, 靳培英, 马林, 王峰来, 沈丹蓓   

  1. 中国医学科学院、中国协和医科大学皮肤病研究所, 南京210042
  • 收稿日期:1995-02-28 修回日期:1995-06-30 出版日期:1996-02-15 发布日期:1996-02-15

Comparative Study of the Methods of Extracting Antigens from Normal Human Epidermis for Detecting Pemphigoid Antibodies

Lin Lin, Jin Peiyin, Ma Lin   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences, Peking Union Medical College, Nanjing 210042
  • Received:1995-02-28 Revised:1995-06-30 Online:1996-02-15 Published:1996-02-15

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摘要： 免疫印迹技术常用于检测大疱性类天疱疮(BP)抗体。充分、完整地提取表皮基底膜中蛋白抗原成分,是运用免疫印迹技术于类天疱疮抗体检查过程中很重要的一部分,直接影响到结果的准确性和完整性。同时简单的提取方法也将方便免疫印迹检测。本文选用三种国外常用的BP抗原提取方法,经用BP血清进行免疫印迹实验,进行比较研究。三种提取制备方法的检测结果基本相同。但是,其中方法1操作较简单,而且提取量高。方法3与获得性大疱性表皮松解症(EBA)抗原的提取制备方法,有许多相似。如需运用免疫印迹技术鉴别BP和EBA时,可采用方法3较方便。

关键词: 抗原, 类天疱疮, 免疫印迹

Abstract: Immunoblotting(IB) technique has been frequently used to detect bullous pemphigoid (BP) antibodies in BP patient.It is important to extract the antigen proteins fully and intactly in the base membrane zone(BMZ) of normal human epidermis.The simple method of extracting antigens will make IB techniqne easy.Three different methods of extracting antigen proteins were compared.Using IB for detecting BP antibodies,it has been found that the results of epidermal extract preparations are basically similar.However,one of these methods (No.1) is more convenient and may obtain more amounts of antigens.Another method(No.3) is identical to that of extracting antigens in dermis for detecting epidermolysis bullosa acquista (EBA) antibodies.Therefore,in using IB technique to distinguish BP from EBA,one can apply method No.3.

Key words: Antigens, Pemphigoid, Immunoblotting