中华皮肤科杂志 ›› 1999, Vol. 32 ›› Issue (3): 165-167.

• 论著 • 上一篇    下一篇

恶性黑素瘤细胞株细胞间粘附分子1的改变及临床意义

夏建新1, 中山树一郎2, 占部和敬2, 母义明3   

  1. 1. 白求恩医科大学第二临床学院皮肤科, 长春130041;
    2. 日本九州大学医学部皮肤科教室;
    3. 北京解放军第三零一医院内分泌科
  • 收稿日期:1998-02-12 修回日期:1998-07-14 出版日期:1999-06-15 发布日期:1999-06-15

The Expression of Intercellular Adhesion Molecule-1 in Human Malignant Melanoma Cell Lines and Its Clinical Significance

XIA Jianxin1, J. Nakayama2, K. Urabe2   

  1. Department of Dermatology, Second Hospital, Norman Bethune Medical University, Changchun 130041
  • Received:1998-02-12 Revised:1998-07-14 Online:1999-06-15 Published:1999-06-15

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摘要： 目的 探讨细胞间粘附分子1(ICAM-1)在恶性黑素瘤细胞株的表达及临床意义。方法 用体外细胞培养方法、逆转录聚合酶链反应技术,观察恶性黑素瘤细胞株MM81在温热及某些细胞因子刺激下,细胞形态、细胞增殖能力及ICAM-1的基因水平变化。结果 温热、γ-干扰素、肿瘤坏死因子α可使细胞形态改变、细胞增殖能力下降、ICAM-1的表达增加,白介素2未能影响细胞形态及增殖能力,也未能使ICAM-1明显改变。结论 恶性黑素瘤细胞株ICAM-1的表达与细胞被破坏有关,同时为温热、γ-干扰素、肿瘤坏死因子-α作为治疗恶性黑素瘤的辅助手段提供了理论依据。

关键词: 黑素瘤, 细胞因子, 细胞间粘附分子1, 逆转录聚合酶链反应

Abstract: Objective To investigate the expression of ICAM-1 in human malignant melanoma (MM)cell lines and its clinical significance.Methods Human MM cell lines MM8.1 were cultured and analyzed by reverse transcription polymerase chain reaction(RT-PCR), the cell shape,cell growth ability and ICAM-1 by mRNA expression under the stimulation of hyperthermia or cytokines were observed.Results Hyperthermia、γ-IFN and TNF-α could change cell shape,reduce cell growth ability and increase the expression of ICAM-1.IL-2 could not influence the cell shape and the expression of ICAM-1.Conclusion The expression of ICAM-1 in MM cell lines may be correlated with the cell destruction,which provides theoretical basis of supplementary therapy for MM with hyperthermia、IFNγ and TNFα.

Key words: Melanoma, Cytokine, Intercellular adhesion molecule-1, Reverse transcription polymerase chain reaction