中华皮肤科杂志 ›› 1999, Vol. 32 ›› Issue (4): 249-251.

• 论著 • 上一篇    下一篇

正常人表皮细胞在增殖与终末分化中DNA的变化

毛祖豪, 梁应权, 鲍永耀, 罗深秋, 王应斌, 张胜方   

  1. 第一军医大学珠江医院, 广州 510282
  • 收稿日期:1998-06-15 修回日期:1998-09-04 出版日期:1999-08-15 发布日期:1999-08-15

Changes of DNA Content of Normal Epidermal Cells During Proliferation and Terminal Differentiation

MAO Zuhao, LIANG Yinquan, BAO Yongyao   

  1. Zhu Jiang Hospital, First Military Medical University, Guangzhou 510282
  • Received:1998-06-15 Revised:1998-09-04 Online:1999-08-15 Published:1999-08-15

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摘要： 目的 探讨正常人表皮细胞在增殖与终末分化中DNA的变化.方法 通过粘附式细胞仪用吖啶橙染色10例正常人皮肤组织切片,测表皮各层细胞DNA综合荧光值、荧光密度、面积.结果 正常表皮细胞DNA综合荧光值、荧光密度、面积,棘层较其他各层均高(P<0.01),其余各层差异无显着性(P>0.05);角质下层仍存在DNA荧光,角质上层DNA荧光极淡.结论 正常人表皮细胞DNA含量、浓度、分布范围以棘层最高,DNA合成最旺盛的部位在棘层,基底细胞DNA合成受到抑制,颗粒层细胞DNA不降解,可能仅发生了变性,而DNA降解的部位在角质层.

关键词: 表皮, DNA

Abstract: Objective To explore the changes of DNA content of normal epidermal cells during proliferation and terminal differentiation. Methods DNA content of human epidermal cells in each layer from normal human skin (10 cases) was determined with adherent cell analysis and sorting cytometer (ACAS570).The tissue sections with 10μm thick were stained with acridine orange (AO), and evaluated by DNA integrated fluorescence value (IV), average fluorescence value (AV), and scanning area (A) of epidermal cells in each layer with ACAS570. Results The results showed that IV,AV, or A were higher in spinous cells than those in cells of other layers(P<0.01), but there was no difference in cells of the other layers (P>0.05). DNA fluorescence was still present in the lower horny layers, and almost disappeared in the upper horny layers. Conclusion The results suggest that total level of DNA content, density or area of distribution are higher or larger in cells of the spinous layer than those of the other layers. These data demonstrate that the high level DNA synthesis takes place in the spinous layer, inhibited in the basal cells, not degraded in the granular layer where DNA may be degenerated, and degraded in the horny layer.

Key words: Epidermis, DNA