中华皮肤科杂志 ›› 1999, Vol. 32 ›› Issue (4): 243-245.

• 论著 • 上一篇    下一篇

成纤维细胞与纤连蛋白粘附及其诱导的酪氨酸磷酸化蛋白在硬皮病原胶原基因表达中的作用

洪微, 廖万清, 孔宪涛, 陈敏亮   

  1. 第二军医大学附属长征医院, 上海 200003
  • 收稿日期:1998-07-16 修回日期:1998-11-26 出版日期:1999-08-15 发布日期:1999-08-15
  • 基金资助:
    国家自然科学基金39670673

Role of Adhesion Between Fibroblasts and Fibronectin and Tyrosine Phosphorylated Proteins Induced by the Adhesion on the Expression of Procollagen in Scleroderma

HONG Wei, LIAO Wanqing, KONG Xiantao   

  1. Department of Dermatology, Changzheng Hospital, Second Military Medical University, Shanghai 200003
  • Received:1998-07-16 Revised:1998-11-26 Online:1999-08-15 Published:1999-08-15

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摘要： 目的 研究硬皮病成纤维细胞与纤连蛋白粘附,以及粘附诱导的酪氨酸磷酸化蛋白在成纤维细胞表达原胶原mRNA中的作用.方法 将硬皮病成纤维细胞置于纤连蛋白包被的培养体系中粘附培养,运用RTPCR、免疫印迹法分别检测原胶原mRNA表达的变化及粘附诱导的酪氨酸磷酸化蛋白;并观察阻断酪氨酸磷酸化后,原胶原mRNA和酪氨酸磷酸化蛋白的变化.结果 硬皮病成纤维细胞与纤连蛋白粘附,可诱导相对分子质量为98000、65000酪氨酸磷酸化蛋白生成,且原胶原proα1(Ⅰ)mRNA的表达显着增加;经酪氨酸激酶抑制剂作用后,伴随着相对分子质量为98000、65000酪氨酸磷酸化蛋白的减少,原胶原proα1(Ⅰ)mRNA显着降低.结论 成纤维细胞与纤连蛋白粘附,在硬皮病原胶原表达增加中具有重要作用,由粘附诱导的酪氨酸磷酸化是其中的一个重要环节.

关键词: 硬皮病, 成纤维细胞, 酪氨酸磷酸化

Abstract: Objective In order to investigate the role of adhesion between fibronectin and fibroblasts from scleroderma as well as tyrosine phosphorylated proteins induced by the adhesion on procollagen mRNA expression. Methods Fibroblasts from lesions of scleroderma were cultured in culture bottles absorbed with fibronectin. The levels of procollagen α1(I) mRNA and tyrosine phosphorylated proteins induced by the adhesion were detected by RT-PCR and immunoblotting, respectively. An inhibitor of tyrosine kinase, herbimycin A, was added to the medium to block the phosphorylation of tyrosine. The changes of procollagen mRNA and tyrosine phosphorylated proteins were further investigated. Results Adhesion between scleroderma fibroblasts and fibronectin not only induced the production of 98 000 and 65 000 tyrosine phosphorylated proteins, but also enhanced obviously the expression of procollagen α1(I) mRNA. When the phosphorylation of tyrosine was blocked, the level of procollagen α1(I) mRNA decreased significantly with the reduction of 98 000, 65 000 tyrosine phosphorylated proteins. Conclusion Adhesion between fibronectin and the fibroblasts plays an important role in enhanced expression of procollagen mRNA in scleroderma. Phosphorylation of tyrosine seems to be a key step in the process of expression of procollagen mRNA.

Key words: Scleroderma, Fibroblasts, Tyrosine phosphorylation