中华皮肤科杂志 ›› 1999, Vol. 32 ›› Issue (4): 223-225.

• 论著 •    下一篇

氨酰基脯氨酸二肽酶缺乏症的诊断和治疗

陈志强1, 刘晓青2, 顾有守1, 申国庆2, 孙建方2, 施惠平2, 徐文严2   

  1. 1. 中国医学科学院、中国协和医科大学皮肤病研究所, 南京 210042;
    2. 中国医学科学院、中国协和医科大学基础医学研究所, 南京 210042
  • 收稿日期:1998-08-23 修回日期:1999-03-12 出版日期:1999-08-15 发布日期:1999-08-15

The Diagnosis and Treatment of Prolidase Deficiency

CHEN Zhiqiang1, LIU Xiaoqing2, GU Youshou1   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences, Peking Union Medical College, Nanjing 210042
  • Received:1998-08-23 Revised:1999-03-12 Online:1999-08-15 Published:1999-08-15

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摘要： 目的 报道一例罕见的氨酰基脯氨酸二肽酶缺乏症及其诊断治疗方法.方法 分别以高效液相色谱法和比色法检测血清与皮肤成纤维细胞中氨酰基脯氨酸二肽酶活性及尿液中甘氨酸-L-脯氨酸二肽的浓度;外用5%脯氨酸、5%甘氨酸乳剂治疗皮肤溃疡.结果 患者血清、尿液及成纤维细胞中氨酰基脯氨酸二肽酶活性均异常,局部治疗促进溃疡愈合.结论 高效液相色谱法和比色法有助于确诊,甘氨酸乳剂治疗有效.

关键词: 氨酰基脯氨酸二肽酶, 成纤维细胞

Abstract: Objective To report a rare case of prolidase deficiency and the methods of diagnosis and treatment. Methods The activity of prolidase (PD) in serum and skin fibroblasts was detected by high-performance liquid chromatography (HPLC) or by colorimetric determination, and the level of glycine L-proline in urine was tested. The skin ulcer was topically treated with an emulsion containing 5% proline and 5% glycine (EPG). Results The activity of PD in the patient's serum, urine and fibroblasts was abnormal, and topical EPG reduced the size of ulcer. Conclusion HPLC and colorimetric determination are useful diagnostic tools, and the topical EPG therapy is helpful.

Key words: Prolidase, Fibroblasts